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AccuAmp Probe qPCR Master Mixes



AccuAmp Probe qPCR Master Mixes are 2X concentrated, ready-to-use reaction cocktails that contain all components, except primers, probe, and template, for real-time quantative PCR. The master mixes provide highly specific and sensitive real-time quantification of genomic DNA or cDNA targets using sequence-specific probe such as Taqman and other dual-labeled probes. For accurate Real-time PCR, it is crucial to reduce/ avoid any nonspecific products and primer–dimers generated during amplification. AccuAmp Probe qPCR Master Mixes promote highly specific annealing of primers and probes to the PCR template through our unique combination of proprietary buffer, stabilizers, and specially modified hot-start Taq DNA polymerase, which prevents any polymerase activity before heat activation, thereby eliminating amplification of nonspecific products.

Real-time PCR Instrument Compatibility

Different Real-time PCR instruments employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match appropriate qPCR reagent to your specific instrument according to the table below. 


Compatible Instruments

AccuAmp Probe qPCR Master Mix/ROX

Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, 7900HT Fast, StepOne, StepOne Plus

AccuAmp Probe qPCR Master Mix/LowROX

Applied Biosystems 7500, 7500 Fast, ViiA 7;
Stratagene Mx3000P, Mx3005P, Mx4000

AccuAmp Probe qPCR Master Mix/Fluorescein

BioRad iCycler iQ, iQ5, MyiQ, MyiQ2

AccuAmp Probe qPCR Master Mix

BioRad/MJ Chromo4, Opticon, MiniOpticon, CFX96, CFX384; Qiagen/Corbett Rotor-Gene;
Roche LightCycler 480; Cepheid SmartCycler;
Mastercycler ep realplex