AccuAmp Probe qPCR Master Mix
Cat. No. |
Size |
Price |
Quantity |
11004-100 |
100 x 25-µl reactions (1.25 ml) |
$69.00 |
|
11004-400 |
400 x 25-µl reactions (4 x 1.25 ml) |
$209.00 |
|
11004-01k |
1000 x 25-µl reactions (12.5 ml) |
$479.00 |
|
11004-02k |
2000 x 25-µl reactions (25 ml) |
$949.00 |
Benefits
- High PCR specificity for use with numerous different targets and templates.
- Outstanding sensitivity with early Ct detection.
- High amplification efficiency over a wide dynamic range.
- Convenient 2x master mix and optimized protocols
Description
AccuAmp Probe qPCR Master Mix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe, and template, for real-time quantative PCR on a variety of instruments that do not require a reference dye. This master mix provides highly specific and sensitive real-time quantification of genomic DNA or cDNA targets using sequence-specific probe such as Taqman and other dual-labeled probes. For accurate Real-time PCR, it is crucial to reduce/ avoid any nonspecific products and primer–dimers generated during amplification. AccuAmp Probe qPCR Master Mix promotes highly specific annealing of primers and probes to the PCR template through our unique combination of proprietary buffer, stabilizers, and specially modified hot-start Taq DNA polymerase, which prevents any polymerase activity before heat activation, thereby eliminating amplification of nonspecific products.
Compatible Instrument
- Roche LightCycler 480
- BioRad CFX96
- BioRad CFX384
- BioRad/MJ Chromo4
- BioRad/MJ Opticon
- BioRad/MJ Opticon 2
- BioRad/MJ MiniOpticon
- Qiagen/Corbett Rotor-Gene Q
- Qiagen/Corbett Rotor-Gene 3000
- Qiagen/Corbett Rotor-Gene 6000
- Eppendorf Mastercycler ep realplex
- Eppendorf Mastercycler ep realplex2 s
- Cepheid SmartCycler
Storage
AccuAmp Probe qPCR Master Mix is stable for 1 year when stored at –20°C in a constant-temperature freezer, protected from light. After thawing, mix thoroughly before using.
Quick-Reference Protocol
1. Thaw AccuAmp Probe qPCR Master Mix (2X), template, primers, and probe. Mix the individual solutions.
2. Prepare a reaction mix.
Component |
Volume/Reaction |
Final Concentration |
AccuAmp Probe qPCR Master |
12.5 µl |
1X |
Forward Primer |
Variable |
400 nM |
Reverse Primer |
Variable |
400 nM |
Probe |
Variable |
100-250 nM |
Template DNA or cDNA |
Variable |
Up to 100 ng/reaction |
Nuclease-free Water |
Variable |
|
Total Reaction Volume |
25µl |
|
3. Mix the reaction mix thoroughly. After sealing each reaction, centrifuge reaction tubes briefly to avoid bubbles.
4. Program your thermal cycler.
Step |
Temperature &Time |
Notes |
PCR initial activation step |
95°C, 10 min |
Hot-start Taq DNA polymerase is activated by this heating step. |
2-step cycling (40 cycles) |
a. 95°C, 15 sec (Denaturation) |
Collect fluorescence data at step b. |
5. Place the PCR tubes or plates in the real-time cycler, and start the cycling program.