AccuAmp Fast SYBR® Green qPCR Master Mixes

Benefits

• High PCR specificity for use with numerous different targets and templates.
• Outstanding sensitivity with early Ct detection.
• High amplification efficiency over a wide dynamic range.
• Faster results with optimized, ready-to-use master mix.

Description

AccuAmp Fast SYBR® Green qPCR Master Mixes are 2X concentrated, ready-to-use reaction cocktails that contain all components, except primers and template, for rapid real-time PCR. The master mixes provide highly specific and sensitive real-time quantification of genomic DNA and cDNA targets using SYBR® Green I, under fast cycling conditions. Since SYBR® Green dye binds to all double-stranded DNA, it is crucial to reduce/ avoid any nonspecific products and primer–dimers generated during amplification. AccuAmp Fast SYBR® Green qPCR Master Mixes promote highly specific annealing of primers to the PCR template through our unique combination of proprietary buffer, stabilizers, and specially modified hot-start Taq DNA polymerase, which prevents any polymerase activity before heat activation, thereby eliminating amplification of nonspecific products.

Real-time PCR Instrument Compatibility

Different Real-time PCR instruments employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match appropriate qPCR reagent to your specific instrument according to the table below.