AccuAmp qPCR Primer Assays are experimentally validated primer pairs for use in SYBR Green-based quantitative real-time PCR. Every AccuAmp qPCR Primer Assay is subject to rigorous experimental validation to guarantee single amplicon of the correct size with high amplification efficiency.

The AccuAmp qPCR Primer Assays have been optimized with use of our AccuAmp SYBR Green qPCR Master Mix and AccuAmp Fast SYBR Green qPCR Master Mix, which are recommended for guaranteed performance. We do not guarantee optimal performance with other sources of master mix, without requiring further optimization studies by the end-user.

AccuAmp qPCR Primer Assatys are available for any gene from human, mouse, and rat.  For custom primer designs for other species, please contact us at support@genescientific.com to get a quote and information.

AccuAmp qPCR Primer Assay can be used on any real-time PCR instrument. Be sure to use an appropriate AccuAmp SYBR Green qPCR master mix that is compatible with your instrument. 

Many genes have more than one transcript due to alternative splicing. The various transcripts are called transcript variants or splice variants. If transcript variants exist for a specific gene, AccuAmp qPCR Primer Assay can detect all transcript variants simultaneously.

The annealing temperature for AccuAmp qPCR Primer Assays should be 60°C. The Use of AccuAmp SYBR Green qPCR Master Mixes and AccuAmp Fast SYBR Green qPCR Master Mixes require a combined annealing/extension step at 60°C.