AccuAmp Probe qPCR Master Mix/ROX
Cat. No. |
Size |
Price |
Quantity |
11001-100 |
100 x 25-µl reactions (1.25 ml) |
$69.00 |
|
11001-400 |
400 x 25-µl reactions (4 x 1.25 ml) |
$209.00 |
|
11001-01k |
1000 x 25-µl reactions (12.5 ml) |
$479.00 |
|
11001-02k |
2000 x 25-µl reactions (25 ml) |
$949.00 |
Benefits
- High PCR specificity for use with numerous different targets and templates.
- Outstanding sensitivity with early Ct detection.
- High amplification efficiency over a wide dynamic range.
- Convenient 2x master mix and optimized protocols
Description
AccuAmp Probe qPCR Master Mix/ ROX is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe, and template, for real-time quantative PCR on Applied Biosystems 7000, 7300, 7700, 7900HT, 7900HT Fast, StepOne, or StepOne Plus instruments. This master mix provides highly specific and sensitive real-time quantification of genomic DNA or cDNA targets using sequence-specific probe such as Taqman and other dual-labeled probes. For accurate Real-time PCR, it is crucial to reduce/ avoid any nonspecific products and primer–dimers generated during amplification. AccuAmp Probe qPCR Master Mix/ ROX promotes highly specific annealing of primers and probes to the PCR template through our unique combination of proprietary buffer, stabilizers, and specially modified hot-start Taq DNA polymerase, which prevents any polymerase activity before heat activation, thereby eliminating amplification of nonspecific products.
Compatible Instrument
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900HT
- Applied Biosystems 7900HT Fast
- Applied Biosystems StepOne
- Applied Biosystems StepOne Plus
Storage
AccuAmp Probe qPCR Master Mix/ ROX is stable for 1 year when stored at –20°C in a constant-temperature freezer, protected from light. After thawing, mix thoroughly before using.
Quick-Reference Protocol
1. Thaw AccuAmp Probe qPCR Master Mix/ROX (2X), template, primers, and probe. Mix the individual solutions.
2. Prepare a reaction mix.
Component |
Volume/Reaction |
Final Concentration |
AccuAmp Probe qPCR Master |
12.5 µl |
1X |
Forward Primer |
Variable |
400 nM |
Reverse Primer |
Variable |
400 nM |
Probe |
Variable |
100-250 nM |
Template DNA or cDNA |
Variable |
Up to 100 ng/reaction |
Nuclease-free Water |
Variable |
|
Total Reaction Volume |
25µl |
|
3. Mix the reaction mix thoroughly. After sealing each reaction, centrifuge reaction tubes briefly to avoid bubbles.
4. Program your thermal cycler.
Step |
Temperature &Time |
Notes |
PCR initial activation step |
95°C, 10 min |
Hot-start Taq DNA polymerase is activated by this heating step. |
2-step cycling (40 cycles) |
a. 95°C, 15 sec (Denaturation) |
Collect fluorescence data at step b. |
5. Place the PCR tubes or plates in the real-time cycler, and start the cycling program.